F2R CLIA Kit for Bovine(Bos taurus) | EIAab.comF2R CLIA Kit for Bovine(Bos taurus) | EIAab.com

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F2R (Gene name),

Proteinase-activated receptor 1 (Protein name ), PAR1_BOVIN from NCBI database.

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General Annotation

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Antigen Annotation

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3D

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Predicted Eptitope

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Vaild Sequence

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Related Databases

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Gene name:

F2R(PAR1);

Protein name:

Proteinase-activated receptor 1(PAR-1);

Alternative:

Thrombin receptor;

Organism:

Bovine (Bos taurus).

General Annotation

Sub Unit:

N/A

Function:

High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis.

Subcellular Location:

Cell membrane Multi-pass membrane protein

Protein Attributes:

Sequence length:

427

Sequence:

50:

MGPRWLLLWA | AGLGLCSPLV | SARTRGPRPG | TDPTNGTLGP | RSFFLRNSND |

100:

GYEQIPLPED | EDSSEGEFTE | DRLSSGNRSS | PPQKSPPGFI | SKSASGYLTS |

150:

AWLTVFIPSV | YTGVFLVSLP | LNIMAVVVFV | LKMKVKKPAV | VYMLHLAAAD |

200:

VLFVCVLPFK | ISYYFSGSDW | RFGSAMCRFV | TAAFYGNMYA | SIMLMTAISV |

250:

DRFLAVVYPI | QSLSWRTLGR | ASFICLAIWA | MAIAGVAPLL | LQEQATQVPG |

300:

LNITACHDVL | NQTLLEGYYS | YYFSAFSAVF | FFVPLTLSTV | SYVSIIRCLS |

350:

SSTVANQNKK | SRALLLSAAV | FCIFILCFGP | TNILLLLHYA | FLSSDPMTEA |

400:

AYFAYLLCVC | VSSISCCIDP | LIYYYASSEC | QRHLFAILHC | KESSDPGSCN |

427:

SSGQLMPSKM | DTCSSNLSSS | LYKKLLT

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

STRING:

9913.ENSBTAP00000026902

UniGene:

KEGG:

Pfam:

String:

Uniprot:

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ELISA Kit for Bovine PAR-1

Cat.:

E0826m

Price:

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Packing:

96T

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ELISA Kit for Bovine PAR-1

Cat.:

E0826h

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Packing:

96T

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ELISA Kit for Bovine PAR-1

Cat.:

E0826r

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96T

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ELISA Kit for Bovine PAR-1

Cat.:

E0826b

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96T

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CLIA Kit for Bovine PAR-1

Cat.:

U0826r

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96T

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CLIA Kit for Bovine PAR-1

Cat.:

U0826h

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Looking for manual.

MSDS:

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Packing:

96T

Range:

Please sign in first.

CLIA Kit for Bovine PAR-1

Cat.:

U0826m

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Looking for manual.

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96T

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CLIA Kit for Bovine PAR-1

Cat.:

U0826b

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MSDS:

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Packing:

96T

Polyclonal Antibody for Bovine PAR-1

Cat.:

P0826Rb-m

Price:

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Packing:

40ug/0.2ml

Polyclonal Antibody for Bovine PAR-1

Polyclonal Antibody for Bovine PAR-1

Cat.:

P0826Rb-r

Price:

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Packing:

40ug/0.2ml

Polyclonal Antibody for Bovine PAR-1

Cat.:

P0826Rb-h

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Packing:

40ug/0.2ml

Monoclonal Antibody for Bovine PAR-1

Monoclonal Antibody for Bovine PAR-1

Monoclonal Antibody for Bovine PAR-1

Monoclonal Antibody for Bovine PAR-1

Protein for Bovine PAR-1

Protein for Bovine PAR-1

Protein for Bovine PAR-1

Protein for Bovine PAR-1

R&D Technical Data

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s

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s

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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Precision

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤7.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤12.1%

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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Recovery

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Recovery was determined by spiking various levels of PAR1 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	96	93-100
Plasma	97	91-103

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Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

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Linearity

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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PAR1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	93-98%	93-100%	94-100%	92-101%
EDTA plasma(n=5)	91-100%	94-99%	90-102%	93-99%
heparin plasma(n=5)	92-99%	91-102%	91-101%	93-100%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

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References

1.

NIH - Mammalian Gene Collection (MGC) project
Submitted (2007-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
strain: Hereford.
tissue: Heart ventricle.

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